The process of IUI is similar to the ovulation induction for timed intercourse till the day of trigger. After trigger depending on the semen analysis and DFI, processing of semen sample will be done- like density gradient methods, MACS, microfluidics. In gradient method or swim up technique and microfluidics, generally, it is advisable to have double IUI to increase the chances of pregnancy, 1st IUI will be 24 hr after the trigger, 2nd IUI will be 48 hr after the trigger. In MACS and microfluidics, DNA fragmented spermatozoa will be filtered and healthy spermatozoa will be inseminated. MACS is advised after documenting ovulation in TVS, generally, a single sitting is enough.